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Its not that bad, if you've ever done any work with mushroom cultures its about the same. Or even canning food at home, not too technical. The technical part is the hormones, and agar formulas. -Make your Agar, or buy a preformulated one. I went the hard way and made mine from Murashige...

Look closely at TC lablets vs. Premium Heart cuts, they have thin stems. I'm finding the same thing with the TC''s I've run, thin stems. My work is based off of the directions in Melinda's work in cannabis TC. Seems to be a common problem. I think its something to do with CO2 exhange, most...

Teas can be adjusted to favor fungal activity or bacteria activity. 1) You'll save money. 2) If you have a microscope you'll see how much more potent live cultures are vs a commercial shelved product. 3) Recycle stuff, I use trim in my teas, bacteria like nitrogen. I get rid of my kids...

My opinion is that vermicompost, compost, or compost tea is the most effective way to get the microbes on the roots for transplant, if you are working from a sterile medium like rockwool. Most commercial soils like promix are pastureized, so they need a bit of active culture to get it going in...

There is some research indicating that high P and K levels is detrimental to Myco. Im going to ramble a bit, but this might helps you understand mycorrhizae better. If you're going to apply a product high in PK, try something plant based. Its more or less suggested that Mycorrhizae...

Earlier in the thread ricksauce said there was a positive TMV result. However he was unsure if it was a false positive or the other 2 were false negatives. Either way, if milk works as broad spectrum virucide, I'm all for it, I would use it as a dip for clones I'll get from socal, but I'll use...

"Washing hands well with soap and water has the best impact on virus removal and inactivation." http://www.gpnmag.com/article/truth-about-milk-and-tmv/ "I just attended a class at a conference yesterday and the speaker showed us research that demonstrated that soap and water (washing hands...

Might as well throw the cut out. Last thing you need is to spread the virus to other plants from scissors. Oddly enough one of the most effective ways to eliminate TMV is with soap and water. If that is the virus responsible for this. Thrips are a vector for viruses, like spotted wilt...

I can help you. This is typical pesticide application math. 1 liter is 1000 ml. 1000 x 1.0 = 1000 1000 x .98 = 980 1000 - 980= 20ml 980 ml water + 20 ml ezwet = 1000 ml solution. 20ml for 2% solution of ezwet. 100-.25 = 99.75 1000 x .9975 = 997.5 997.5-1000 = 2.5 2.5 ml ezwet + 997.5ml...

Its possible that there is no relation with smoking and dudding. Its known that contact with tobacco is a known vector of pathogens it is unclear if TMV is the cause of dudding. There is alot of doubt.. Due to the fact that ricksauce says that only 1 of the 3 tested positive for TMV and he...

I had a hard time reading those abstracts.. or at least trying to figure out how to apply it as part of a eradication plan. I did however find a simplified version. It discusses vector control, efficacy.. and some interesting info on the virus. And it does include references to prior...

@ ricksauce. I know you're working with the virus theory.. but has there been any work suggesting abiotic factors like herbicide damage?.. Neat stuff about using PCR for virus ID, I'll have to talk to my consultant about that, we were talking about using PCR for genetic ID, but it would...

Its possible that it isn't a virus.. An abiotic condition like you mentioned could cause a dud.

Would you mind looking at this formula? Reversing Formula, to make pollen from females. Silver Nitrate + Sodium Thiosulfate Pentahydrate, in H20. .5 Grams of silver nitrate in 1000ml water. (Part A) 3.9 Grams of sodium thiosulfate pentahydrate dissolved in 1000ml water. (Part B) Mixed A+B...

I'd like to eliminate that possiblity of tobacco use.. but I think its more like you said. I wonder if the cuts over time loose their ablity to fight a viral infection.. Just throwing it out there.. GG4/GSC geeze.. Ive done hundreds of clippings I'm worried about the dud problem. Perhaps...

Read into it however you want.. I'd like to eliminate any possible transmission TMV that would cause duding in my clones, or stop any behaviors that could infect a crop... My point is why risk the possiblity of infection.. Fuck tobacco, I'd rather smoke a bowl in the garden..

The smoke isn't how TMV is spread.. I think its a legitimate concern for say a person that dips, handles leaves or has contact with TMV infected crop, then touches cannabis crop. The point is to reduce the possibility of TMV infection to the crop, your stance is literally identical to the...

Good stuff.. thank you. Not saying that TMV is the cause.. but if it tests positive for it. Ive kinda argued this over the years with smokers and handling cannabis.. Its a possible link.. I'll have to read the links provided..

Yeah, its an interesting thread.. Again with the name, its called MTF.. not ATF not MVTF. If MTF is so badass, why is it so hard to find?.. Think about it... Like I've said before, MTF just cant stand up against the modern hybrids. A buddy of mine talked about this the other day...

Alaska pot regulators approve allowing marijuana use at certain retail stores http://www.ktuu.com/news/news/alaska-pot-regulators-approve-allowing-marijuana-use-at-certain-retail-stores/36581370 There it is.. "Anchordam" I coined the word.. :) Dont want to watch the video?.. In summary...